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-Structure paper
タイトル | The structure of the phage T4 DNA packaging motor suggests a mechanism dependent on electrostatic forces. |
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ジャーナル・号・ページ | Cell, Vol. 135, Issue 7, Page 1251-1262, Year 2008 |
掲載日 | 2008年12月26日 |
著者 | Siyang Sun / Kiran Kondabagil / Bonnie Draper / Tanfis I Alam / Valorie D Bowman / Zhihong Zhang / Shylaja Hegde / Andrei Fokine / Michael G Rossmann / Venigalla B Rao / |
PubMed 要旨 | Viral genomes are packaged into "procapsids" by powerful molecular motors. We report the crystal structure of the DNA packaging motor protein, gene product 17 (gp17), in bacteriophage T4. The ...Viral genomes are packaged into "procapsids" by powerful molecular motors. We report the crystal structure of the DNA packaging motor protein, gene product 17 (gp17), in bacteriophage T4. The structure consists of an N-terminal ATPase domain, which provides energy for compacting DNA, and a C-terminal nuclease domain, which terminates packaging. We show that another function of the C-terminal domain is to translocate the genome into the procapsid. The two domains are in close contact in the crystal structure, representing a "tensed state." A cryo-electron microscopy reconstruction of the T4 procapsid complexed with gp17 shows that the packaging motor is a pentamer and that the domains within each monomer are spatially separated, representing a "relaxed state." These structures suggest a mechanism, supported by mutational and other data, in which electrostatic forces drive the DNA packaging by alternating between tensed and relaxed states. Similar mechanisms may occur in other molecular motors. |
リンク | Cell / PubMed:19109896 |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.16 - 34.0 Å |
構造データ | EMDB-1572: The T4 packaging motor, T4 procapsid with large terminase gp17 bound EMDB-1573: PDB-3c6a: PDB-3c6h: PDB-3cpe: |
化合物 | ChemComp-MG: ChemComp-HOH: ChemComp-PO4: ChemComp-NA: |
由来 |
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キーワード | VIRAL PROTEIN / terminase nuclease / HYDROLASE / large terminase / Alternative initiation / ATP-binding / DNA-binding / Nuclease / Nucleotide-binding / pentameric motor / DNA packaging |