+検索条件
-Structure paper
タイトル | The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 4681, Year 2023 |
掲載日 | 2023年8月4日 |
著者 | Ziyu Zhao / Irene Vercellino / Jana Knoppová / Roman Sobotka / James W Murray / Peter J Nixon / Leonid A Sazanov / Josef Komenda / |
PubMed 要旨 | Robust oxygenic photosynthesis requires a suite of accessory factors to ensure efficient assembly and repair of the oxygen-evolving photosystem two (PSII) complex. The highly conserved Ycf48 assembly ...Robust oxygenic photosynthesis requires a suite of accessory factors to ensure efficient assembly and repair of the oxygen-evolving photosystem two (PSII) complex. The highly conserved Ycf48 assembly factor binds to the newly synthesized D1 reaction center polypeptide and promotes the initial steps of PSII assembly, but its binding site is unclear. Here we use cryo-electron microscopy to determine the structure of a cyanobacterial PSII D1/D2 reaction center assembly complex with Ycf48 attached. Ycf48, a 7-bladed beta propeller, binds to the amino-acid residues of D1 that ultimately ligate the water-oxidising MnCaO cluster, thereby preventing the premature binding of Mn and Ca ions and protecting the site from damage. Interactions with D2 help explain how Ycf48 promotes assembly of the D1/D2 complex. Overall, our work provides valuable insights into the early stages of PSII assembly and the structural changes that create the binding site for the MnCaO cluster. |
リンク | Nat Commun / PubMed:37542031 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.9 - 3.15 Å |
構造データ | EMDB-15522, PDB-8am5: EMDB-15618, PDB-8asl: EMDB-15621, PDB-8asp: |
化合物 | ChemComp-FE2: ChemComp-CLA: ChemComp-PHO: ChemComp-BCR: ChemComp-HEM: ChemComp-CL0: ChemComp-PQN: ChemComp-SF4: ChemComp-LHG: ChemComp-LMG: ChemComp-45D: ChemComp-ECH: ChemComp-EQ3: ChemComp-ZEX: ChemComp-LMT: |
由来 |
|
キーワード | PHOTOSYNTHESIS / photosystem / assembly factor / membrane protein |