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-Structure paper
タイトル | Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach. |
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ジャーナル・号・ページ | Front Mol Biosci, Vol. 9, Page 1044509, Year 2022 |
掲載日 | 2022年11月10日 |
著者 | Gabriele Bongiovanni / Oliver F Harder / Marcel Drabbels / Ulrich J Lorenz / |
PubMed 要旨 | We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow ...We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow dynamics of the embedded particles to occur. Once the laser beam is switched off, the sample revitrifies within just a few microseconds, trapping the particles in their transient configurations, which can subsequently be imaged to obtain a snap shot of the dynamics at this point in time. While we have previously performed such experiments with a modified transmission electron microscope, we here demonstrate a simpler implementation that uses an optical microscope. We believe that this will make our technique more easily accessible and hope that it will encourage other groups to apply microsecond time-resolved cryo-EM to study the fast dynamics of a variety of proteins. |
リンク | Front Mol Biosci / PubMed:36438663 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 1.47 - 1.63 Å |
構造データ | EMDB-15619: Mouse heavy chain apoferritin in plunge-frozen vitreous ice EMDB-15620: Mouse heavy chain apoferritin in vitreous ice after laser-melting and revitrification |
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