EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryoelectron tomography of HIV-1 envelope spikes: further evidence for tripod-like legs.
ジャーナル・号・ページ	PLoS Pathog, Vol. 4, Issue 11, Page e1000203, Year 2008
掲載日	2008年11月14日
著者	Ping Zhu / Hanspeter Winkler / Elena Chertova / Kenneth A Taylor / Kenneth H Roux /
PubMed 要旨	A detailed understanding of the morphology of the HIV-1 envelope (Env) spike is key to understanding viral pathogenesis and for informed vaccine design. We have previously presented a cryoelectron ...A detailed understanding of the morphology of the HIV-1 envelope (Env) spike is key to understanding viral pathogenesis and for informed vaccine design. We have previously presented a cryoelectron microscopic tomogram (cryoET) of the Env spikes on SIV virions. Several structural features were noted in the gp120 head and gp41 stalk regions. Perhaps most notable was the presence of three splayed legs projecting obliquely from the base of the spike head toward the viral membrane. Subsequently, a second 3D image of SIV spikes, also obtained by cryoET, was published by another group which featured a compact vertical stalk. We now report the cryoET analysis of HIV-1 virion-associated Env spikes using enhanced analytical cryoET procedures. More than 2,000 Env spike volumes were initially selected, aligned, and sorted into structural classes using algorithms that compensate for the "missing wedge" and do not impose any symmetry. The results show varying morphologies between structural classes: some classes showed trimers in the head domains; nearly all showed two or three legs, though unambiguous three-fold symmetry was not observed either in the heads or the legs. Subsequently, clearer evidence of trimeric head domains and three splayed legs emerged when head and leg volumes were independently aligned and classified. These data show that HIV-1, like SIV, also displays the tripod-like leg configuration, and, unexpectedly, shows considerable gp41 leg flexibility/heteromorphology. The tripod-like model for gp41 is consistent with, and helps explain, many of the unique biophysical and immunological features of this region.
リンク	PLoS Pathog / PubMed:19008954 / PubMed Central
手法	EM (トモグラフィー) / EM (サブトモグラム平均)
構造データ	EMDB-1513: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1514: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1515: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1516: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1517: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1518: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1519: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1520: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1521: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1522: Cryoelectron tomography of HIV-1 envelope spikes 手法: EM (トモグラフィー) EMDB-1596: HIV-1 Env spike maps using various alignment and classfication combinations 手法: EM (サブトモグラム平均)