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-Structure paper
タイトル | Sensing of individual stalled 80S ribosomes by Fap1 for nonfunctional rRNA turnover. |
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ジャーナル・号・ページ | Mol Cell, Vol. 82, Issue 18, Page 3424-33437.e8, Year 2022 |
掲載日 | 2022年9月15日 |
著者 | Sihan Li / Ken Ikeuchi / Misaki Kato / Robert Buschauer / Takato Sugiyama / Shungo Adachi / Hideo Kusano / Tohru Natsume / Otto Berninghausen / Yoshitaka Matsuo / Thomas Becker / Roland Beckmann / Toshifumi Inada / |
PubMed 要旨 | Cells can respond to stalled ribosomes by sensing ribosome collisions and employing quality control pathways. How ribosome stalling is resolved without collisions, however, has remained elusive. ...Cells can respond to stalled ribosomes by sensing ribosome collisions and employing quality control pathways. How ribosome stalling is resolved without collisions, however, has remained elusive. Here, focusing on noncolliding stalling exhibited by decoding-defective ribosomes, we identified Fap1 as a stalling sensor triggering 18S nonfunctional rRNA decay via polyubiquitination of uS3. Ribosome profiling revealed an enrichment of Fap1 at the translation initiation site but also an association with elongating individual ribosomes. Cryo-EM structures of Fap1-bound ribosomes elucidated Fap1 probing the mRNA simultaneously at both the entry and exit channels suggesting an mRNA stasis sensing activity, and Fap1 sterically hinders the formation of canonical collided di-ribosomes. Our findings indicate that individual stalled ribosomes are the potential signal for ribosome dysfunction, leading to accelerated turnover of the ribosome itself. |
リンク | Mol Cell / PubMed:36113412 |
手法 | EM (単粒子) |
解像度 | 2.4 - 5.8 Å |
構造データ | EMDB-14990, PDB-7zw0: EMDB-14992: FAP-80S Complex - Non-rotated state with empty A site EMDB-14994: FAP-80S Complex - Non-rotated state with eRF1-ABCE1 EMDB-15655: A Consensus Map of Yeast FAP-80S Complex in Rotated State. EMDB-15656: Yeast FAP-80S Complex in Rotated State - Body 1 - 60S subunit EMDB-15657: Yeast FAP-80S in rotated state - Body 3 - 40S head and Fap1-Fpr1 EMDB-15658: Local refined map of Fap1-arm and Yil161w/Smu2 of yeast FAP-80S complex in rotated state. EMDB-15659: Local refined map of Fap1 RING-finger domain with partial 40S subunit of yeast FAP-80S complex in rotated state. EMDB-15660: Yeast FAP-80S Complex in Rotated State - Body 2 - 40S body with Fap1 R3H domain. |
化合物 | ChemComp-MG: ChemComp-SPD: ChemComp-ZN: |
由来 |
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キーワード | RIBOSOME / NRD / Ubiquitination / E3 ligase / Quality Control System |