EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanisms of DNA opening revealed in AAA+ transcription complex structures.
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 51, Page eadd3479, Year 2022
掲載日	2022年12月21日
著者	Fuzhou Ye / Forson Gao / Xiaojiao Liu / Martin Buck / Xiaodong Zhang /
PubMed 要旨	Gene transcription is carried out by RNA polymerase (RNAP) and requires the conversion of the initial closed promoter complex, where DNA is double stranded, to a transcription-competent open promoter ...Gene transcription is carried out by RNA polymerase (RNAP) and requires the conversion of the initial closed promoter complex, where DNA is double stranded, to a transcription-competent open promoter complex, where DNA is opened up. In bacteria, RNAP relies on σ factors for its promoter specificities. Using a special form of sigma factor (σ), which forms a stable closed complex and requires its activator that belongs to the AAA+ ATPases (ATPases associated with diverse cellular activities), we obtained cryo-electron microscopy structures of transcription initiation complexes that reveal a previously unidentified process of DNA melting opening. The σ amino terminus threads through the locally opened up DNA and then becomes enclosed by the AAA+ hexameric ring in the activator-bound intermediate complex. Our structures suggest how ATP hydrolysis by the AAA+ activator could remove the σ inhibition while helping to open up DNA, using σ amino-terminal peptide as a pry bar.
リンク	Sci Adv / PubMed:36542713 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 4.5 Å
構造データ	14171 7qv9 EMDB-14171, PDB-7qv9: CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-14172: CryoEM structure of bacterial transcription intermediate complex mediated by activator protein PspF 手法: EM (単粒子) / 解像度: 4.2 Å 14190 7qwp EMDB-14190, PDB-7qwp: CryoEM structure of bacterial transcription close complex (RPc) 手法: EM (単粒子) / 解像度: 3.4 Å 14200 7qxi EMDB-14200, PDB-7qxi: Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-14206: Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-14207: Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-14208: Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex 手法: EM (単粒子) / 解像度: 4.3 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-AF3*: ALUMINUM FLUORIDE / トリフルオロアルミニウム
由来	escherichia coli k-12 (大腸菌) klebsiella pneumoniae (肺炎桿菌)
キーワード	TRANSCRIPTION / RNA polymerase / AAA protein / transcription regulation / cryoEM / Cryo EM / transcription close complex / sigma54