+検索条件
-Structure paper
タイトル | Membrane Remodeling and Matrix Dispersal Intermediates During Mammalian Acrosomal Exocytosis. |
---|---|
ジャーナル・号・ページ | Front Cell Dev Biol, Vol. 9, Page 765673, Year 2021 |
掲載日 | 2021年12月10日 |
著者 | Miguel Ricardo Leung / Ravi Teja Ravi / Bart M Gadella / Tzviya Zeev-Ben-Mordehai / |
PubMed 要旨 | To become fertilization-competent, mammalian sperm must undergo a complex series of biochemical and morphological changes in the female reproductive tract. These changes, collectively called ...To become fertilization-competent, mammalian sperm must undergo a complex series of biochemical and morphological changes in the female reproductive tract. These changes, collectively called capacitation, culminate in the exocytosis of the acrosome, a large vesicle overlying the nucleus. Acrosomal exocytosis is not an all-or-nothing event but rather a regulated process in which vesicle cargo disperses gradually. However, the structural mechanisms underlying this controlled release remain undefined. In addition, unlike other exocytotic events, fusing membranes are shed as vesicles; the cell thus loses the entire anterior two-thirds of its plasma membrane and yet remains intact, while the remaining nonvesiculated plasma membrane becomes fusogenic. Precisely how cell integrity is maintained throughout this drastic vesiculation process is unclear, as is how it ultimately leads to the acquisition of fusion competence. Here, we use cryoelectron tomography to visualize these processes in unfixed, unstained, fully hydrated sperm. We show that paracrystalline structures within the acrosome disassemble during capacitation and acrosomal exocytosis, representing a plausible mechanism for gradual dispersal of the acrosomal matrix. We find that the architecture of the sperm head supports an atypical membrane fission-fusion pathway that maintains cell integrity. Finally, we detail how the acrosome reaction transforms both the micron-scale topography and the nanoscale protein landscape of the sperm surface, thus priming the sperm for fertilization. |
リンク | Front Cell Dev Biol / PubMed:34957098 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 35.0 Å |
構造データ | EMDB-13877: subtomogram average of paracrystalline patches from the acrosome of capacitated boar sperm |
由来 |
|