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-Structure paper
タイトル | Helical ordering of envelope-associated proteins and glycoproteins in respiratory syncytial virus. |
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ジャーナル・号・ページ | EMBO J, Vol. 41, Issue 3, Page e109728, Year 2022 |
掲載日 | 2022年2月1日 |
![]() | Michaela J Conley / Judith M Short / Andrew M Burns / James Streetley / Joshua Hutchings / Saskia E Bakker / B Joanne Power / Hussain Jaffery / Joanne Haney / Giulia Zanetti / Pablo R Murcia / Murray Stewart / Rachel Fearns / Swetha Vijayakrishnan / David Bhella / ![]() ![]() |
PubMed 要旨 | Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image ...Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three-dimensional reconstruction, we show that there is extensive helical ordering of the envelope-associated proteins and glycoproteins of RSV filamentous virions. We calculated a 16 Å resolution sub-tomogram average of the matrix protein (M) layer that forms an endoskeleton below the viral envelope. These data define a helical lattice of M-dimers, showing how M is oriented relative to the viral envelope. Glycoproteins that stud the viral envelope were also found to be helically ordered, a property that was coordinated by the M-layer. Furthermore, envelope glycoproteins clustered in pairs, a feature that may have implications for the conformation of fusion (F) glycoprotein epitopes that are the principal target for vaccine and monoclonal antibody development. We also report the presence, in authentic virus infections, of N-RNA rings packaged within RSV virions. These data provide molecular insight into the organisation of the virion and the mechanism of its assembly. |
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手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 16.0 Å |
構造データ | ![]() EMDB-13855: ![]() EMDB-13856: Tomogram of respiratory syncytial virus filamentous virion |
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