EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	E-site drug specificity of the human pathogen ribosome.
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 21, Page eabn1062, Year 2022
掲載日	2022年5月27日
著者	Yury Zgadzay / Olga Kolosova / Artem Stetsenko / Cheng Wu / David Bruchlen / Konstantin Usachev / Shamil Validov / Lasse Jenner / Andrey Rogachev / Gulnara Yusupova / Matthew S Sachs / Albert Guskov / Marat Yusupov /
PubMed 要旨	is a widespread commensal fungus with substantial pathogenic potential and steadily increasing resistance to current antifungal drugs. It is known to be resistant to cycloheximide (CHX) that binds ... is a widespread commensal fungus with substantial pathogenic potential and steadily increasing resistance to current antifungal drugs. It is known to be resistant to cycloheximide (CHX) that binds to the E-transfer RNA binding site of the ribosome. Because of lack of structural information, it is neither possible to understand the nature of the resistance nor to develop novel inhibitors. To overcome this issue, we determined the structure of the vacant 80 ribosome at 2.3 angstroms and its complexes with bound inhibitors at resolutions better than 2.9 angstroms using cryo-electron microscopy. Our structures reveal how a change in a conserved amino acid in ribosomal protein eL42 explains CHX resistance in and forms a basis for further antifungal drug development.
リンク	Sci Adv / PubMed:35613268 / PubMed Central
手法	EM (単粒子)
解像度	2.32 - 2.77 Å
構造データ	13737 7pzy EMDB-13737: Cryo-EM map of the vacant Candida albicans 80S ribosome PDB-7pzy: Structure of the vacant Candida albicans 80S ribosome 手法: EM (単粒子) / 解像度: 2.32 Å 13741 7q08 EMDB-13741, PDB-7q08: Structure of Candida albicans 80S ribosome in complex with cycloheximide 手法: EM (単粒子) / 解像度: 2.56 Å 13744 7q0f EMDB-13744: Cryo-EM map of Candida albicans 80S ribosome in complex with phyllanthoside PDB-7q0f: Structure of Candida albicans 80S ribosome in complex with phyllanthoside 手法: EM (単粒子) / 解像度: 2.64 Å 13749 7q0p EMDB-13749: Cryo-EM map of the Candida albicans 80S ribosome in complex with anisomycin PDB-7q0p: Structure of the Candida albicans 80S ribosome in complex with anisomycin 手法: EM (単粒子) / 解像度: 2.77 Å 13750 7q0r EMDB-13750: Cryo-EM map of the Candida albicans 80S ribosome in complex with blasticidin s PDB-7q0r: Structure of the Candida albicans 80S ribosome in complex with blasticidin s 手法: EM (単粒子) / 解像度: 2.67 Å
化合物	ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-PUT: 1,4-DIAMINOBUTANE / プトレシン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-3HE: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione / シクロヘキシミド ChemComp-3K5: 3-O-acetyl-2-O-(3-O-acetyl-6-deoxy-beta-D-glucopyranosyl)-6-deoxy-1-O-{[(2R,2'S,3a'R,4''S,5''R,6'S,7a'S)-5''-methyl-4''-{[(2E)-3-phenylprop-2-enoyl]oxy}decahydrodispiro[oxirane-2,3'-[1]benzofuran-2',2''-pyran]-6'-yl]carbonyl}-beta-D-glucopyranose ChemComp-ANM: ANISOMYCIN / アニソマイシン / 抗生剤YM ChemComp-BLS: BLASTICIDIN S / ブラストサイジンS / 抗生剤YM
由来	candida albicans sc5314 (酵母) candida albicans (酵母)
キーワード	RIBOSOME / Candida albicans / cryo-EM / cycloheximide / drug design / phyllanthoside / anisomycin / peptidyl transferase center / blasticidin s