EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 104, Issue 14, Page 5788-5793, Year 2007
掲載日	2007年4月3日
著者	Robert J C Gilbert / Yulya Gordiyenko / Tobias von der Haar / Andreas F-P Sonnen / Gregor Hofmann / Maria Nardelli / David I Stuart / John E G McCarthy /
PubMed 要旨	In the process of protein synthesis, the small (40S) subunit of the eukaryotic ribosome is recruited to the capped 5' end of the mRNA, from which point it scans along the 5' untranslated region in ...In the process of protein synthesis, the small (40S) subunit of the eukaryotic ribosome is recruited to the capped 5' end of the mRNA, from which point it scans along the 5' untranslated region in search of a start codon. However, the 40S subunit alone is not capable of functional association with cellular mRNA species; it has to be prepared for the recruitment and scanning steps by interactions with a group of eukaryotic initiation factors (eIFs). In budding yeast, an important subset of these factors (1, 2, 3, and 5) can form a multifactor complex (MFC). Here, we describe cryo-EM reconstructions of the 40S subunit, of the MFC, and of 40S complexes with MFC factors plus eIF1A. These studies reveal the positioning of the core MFC on the 40S subunit, and show how eIF-binding induces mobility in the head and platform and reconfigures the head-platform-body relationship. This is expected to increase the accessibility of the mRNA channel, thus enabling the 40S subunit to convert to a recruitment-competent state.
リンク	Proc Natl Acad Sci U S A / PubMed:17389391 / PubMed Central
手法	EM (単粒子)
解像度	14.0 - 30.0 Å
構造データ	EMDB-1327: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-1328: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-1329: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-1330: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-1331: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-1332: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. 手法: EM (単粒子) / 解像度: 14.0 Å
由来	Saccharomyces cerevisiae (パン酵母)