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-Structure paper
タイトル | Structure of immature West Nile virus. |
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ジャーナル・号・ページ | J Virol, Vol. 81, Issue 11, Page 6141-6145, Year 2007 |
掲載日 | 2007年3月21日 |
![]() | Ying Zhang / Bärbel Kaufmann / Paul R Chipman / Richard J Kuhn / Michael G Rossmann / ![]() |
PubMed 要旨 | The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by ...The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses. |
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手法 | EM (単粒子) |
解像度 | 24.0 Å |
構造データ | |
由来 |
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![]() | VIRUS / EM immature flavivirus West Nile / icosahedral virus |