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-Structure paper
タイトル | The structure of a dimeric form of SARS-CoV-2 polymerase. |
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ジャーナル・号・ページ | Commun Biol, Vol. 4, Issue 1, Page 999, Year 2021 |
掲載日 | 2021年8月24日 |
著者 | Florian A Jochheim / Dimitry Tegunov / Hauke S Hillen / Jana Schmitzová / Goran Kokic / Christian Dienemann / Patrick Cramer / |
PubMed 要旨 | The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed of the catalytic ...The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed of the catalytic subunit nsp12, two copies of subunit nsp8, and one copy of subunit nsp7. Here we report an alternative, dimeric form of the enzyme and resolve its structure at 5.5 Å resolution. In this structure, the two RdRps contain only one copy of nsp8 each and dimerize via their nsp7 subunits to adopt an antiparallel arrangement. We speculate that the RdRp dimer facilitates template switching during production of sub-genomic RNAs. |
リンク | Commun Biol / PubMed:34429502 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 5.5 Å |
構造データ | EMDB-13116, PDB-7oyg: |
化合物 | ChemComp-ZN: |
由来 |
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キーワード | VIRAL PROTEIN / SARS-CoV-2 / Polymerase / Transcription / Replication / RNA / Dimer |