EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A distinct assembly pathway of the human 39S late pre-mitoribosome.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 4544, Year 2021
掲載日	2021年7月27日
著者	Jingdong Cheng / Otto Berninghausen / Roland Beckmann /
PubMed 要旨	Assembly of the mitoribosome is largely enigmatic and involves numerous assembly factors. Little is known about their function and the architectural transitions of the pre-ribosomal intermediates. ...Assembly of the mitoribosome is largely enigmatic and involves numerous assembly factors. Little is known about their function and the architectural transitions of the pre-ribosomal intermediates. Here, we solve cryo-EM structures of the human 39S large subunit pre-ribosomes, representing five distinct late states. Besides the MALSU1 complex used as bait for affinity purification, we identify several assembly factors, including the DDX28 helicase, MRM3, GTPBP10 and the NSUN4-mTERF4 complex, all of which keep the 16S rRNA in immature conformations. The late transitions mainly involve rRNA domains IV and V, which form the central protuberance, the intersubunit side and the peptidyltransferase center of the 39S subunit. Unexpectedly, we find deacylated tRNA in the ribosomal E-site, suggesting a role in 39S assembly. Taken together, our study provides an architectural inventory of the distinct late assembly phase of the human 39S mitoribosome.
リンク	Nat Commun / PubMed:34315873 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 5.7 Å
構造データ	12919 7oi6 EMDB-12919, PDB-7oi6: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 1 手法: EM (単粒子) / 解像度: 5.7 Å 12920 7oi7 EMDB-12920, PDB-7oi7: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 2 手法: EM (単粒子) / 解像度: 3.5 Å 12921 7oi8 EMDB-12921, PDB-7oi8: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3A 手法: EM (単粒子) / 解像度: 3.5 Å 12922 7oi9 EMDB-12922, PDB-7oi9: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3B 手法: EM (単粒子) / 解像度: 3.3 Å 12923 7oia EMDB-12923, PDB-7oia: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3C 手法: EM (単粒子) / 解像度: 3.2 Å 12924 7oib EMDB-12924, PDB-7oib: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3D 手法: EM (単粒子) / 解像度: 3.3 Å 12925 7oic EMDB-12925, PDB-7oic: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 4 手法: EM (単粒子) / 解像度: 3.1 Å 12926 7oid EMDB-12926, PDB-7oid: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5A 手法: EM (単粒子) / 解像度: 3.7 Å 12927 7oie EMDB-12927, PDB-7oie: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5B 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸 ChemComp-C: CYTIDINE-5'-MONOPHOSPHATE / CMP ChemComp-A: ADENOSINE-5'-MONOPHOSPHATE / AMP ChemComp-SAM: S-ADENOSYLMETHIONINE
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	RIBOSOME / 39S mitoribosome / ribosome biogenesis