EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Stepwise maturation of the peptidyl transferase region of human mitoribosomes.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 3671, Year 2021
掲載日	2021年6月16日
著者	Tea Lenarčič / Mateusz Jaskolowski / Marc Leibundgut / Alain Scaiola / Tanja Schönhut / Martin Saurer / Richard G Lee / Oliver Rackham / Aleksandra Filipovska / Nenad Ban /
PubMed 要旨	Mitochondrial ribosomes are specialized for the synthesis of membrane proteins responsible for oxidative phosphorylation. Mammalian mitoribosomes have diverged considerably from the ancestral ...Mitochondrial ribosomes are specialized for the synthesis of membrane proteins responsible for oxidative phosphorylation. Mammalian mitoribosomes have diverged considerably from the ancestral bacterial ribosomes and feature dramatically reduced ribosomal RNAs. The structural basis of the mammalian mitochondrial ribosome assembly is currently not well understood. Here we present eight distinct assembly intermediates of the human large mitoribosomal subunit involving seven assembly factors. We discover that the NSUN4-MTERF4 dimer plays a critical role in the process by stabilizing the 16S rRNA in a conformation that exposes the functionally important regions of rRNA for modification by the MRM2 methyltransferase and quality control interactions with the conserved mitochondrial GTPase MTG2 that contacts the sarcin-ricin loop and the immature active site. The successive action of these factors leads to the formation of the peptidyl transferase active site of the mitoribosome and the folding of the surrounding rRNA regions responsible for interactions with tRNAs and the small ribosomal subunit.
リンク	Nat Commun / PubMed:34135320 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.5 Å
構造データ	12845 7odr EMDB-12845, PDB-7odr: State A of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 2.9 Å 12846 7ods EMDB-12846, PDB-7ods: State B of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.1 Å 12847 7odt EMDB-12847, PDB-7odt: State C of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-12848: State A0 of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-12849: State C0 of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-12850: State D0' of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-12851: State D0'' of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-12852: State D of the human mitoribosomal large subunit assembly intermediate 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-PM8: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE ChemComp-SAM: S-ADENOSYLMETHIONINE ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-N: ANY 5'-MONOPHOSPHATE NUCLEOTIDE ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / mitoribosome / assembly intermediate / large subunit / LSU / mt-LSU