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-Structure paper
タイトル | The structure of an infectious P22 virion shows the signal for headful DNA packaging. |
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ジャーナル・号・ページ | Science, Vol. 312, Issue 5781, Page 1791-1795, Year 2006 |
掲載日 | 2006年6月23日 |
著者 | Gabriel C Lander / Liang Tang / Sherwood R Casjens / Eddie B Gilcrease / Peter Prevelige / Anton Poliakov / Clinton S Potter / Bridget Carragher / John E Johnson / |
PubMed 要旨 | Bacteriophages, herpesviruses, and other large double-stranded DNA (dsDNA) viruses contain molecular machines that pump DNA into preassembled procapsids, generating internal capsid pressures ...Bacteriophages, herpesviruses, and other large double-stranded DNA (dsDNA) viruses contain molecular machines that pump DNA into preassembled procapsids, generating internal capsid pressures exceeding, by 10-fold, that of bottled champagne. A 17 angstrom resolution asymmetric reconstruction of the infectious P22 virion reveals that tightly spooled DNA about the portal dodecamer forces a conformation that is significantly different from that observed in isolated portals assembled from ectopically expressed protein. We propose that the tight dsDNA spooling activates the switch that signals the headful chromosome packing density to the particle exterior. |
リンク | Science / PubMed:16709746 |
手法 | EM (単粒子) |
解像度 | 17.0 Å |
構造データ | EMDB-1220: |