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-Structure paper
タイトル | Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli. |
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ジャーナル・号・ページ | EMBO J, Vol. 40, Issue 17, Page e106887, Year 2021 |
掲載日 | 2021年9月1日 |
![]() | Brajabandhu Pradhan / Janine Liedtke / Mike Sleutel / Toril Lindbäck / Ephrem Debebe Zegeye / Kristin O Sullivan / Ann-Katrin Llarena / Ola Brynildsrud / Marina Aspholm / Han Remaut / ![]() ![]() |
PubMed 要旨 | Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity ...Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by β-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores. |
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手法 | EM (らせん対称) |
解像度 | 3.0 - 3.41 Å |
構造データ | EMDB-11591, PDB-7a02: ![]() EMDB-11592: |
由来 |
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![]() | UNKNOWN FUNCTION / Pilli / Endospore / Gram-Positive / Helical reconstruction / Disulphide bridge |