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-Structure paper
タイトル | The Halastavi árva Virus Intergenic Region IRES Promotes Translation by the Simplest Possible Initiation Mechanism. |
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ジャーナル・号・ページ | Cell Rep, Vol. 33, Issue 10, Page 108476, Year 2020 |
掲載日 | 2020年12月8日 |
著者 | Irina S Abaeva / Quentin Vicens / Anthony Bochler / Heddy Soufari / Angelita Simonetti / Tatyana V Pestova / Yaser Hashem / Christopher U T Hellen / |
PubMed 要旨 | Dicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the ...Dicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the elongation cycle via formation of IRES/80S complexes resembling the pre-translocation state. eEF2 then translocates the [codon-anticodon]-mimicking pseudoknot I (PKI) from ribosomal A sites to P sites, bringing the first sense codon into the decoding center. Halastavi árva virus (HalV) contains an IGR that is related to previously described IGR IRESs but lacks domain 2, which enables these IRESs to bind to individual 40S ribosomal subunits. By using in vitro reconstitution and cryoelectron microscopy (cryo-EM), we now report that the HalV IGR IRES functions by the simplest initiation mechanism that involves binding to 80S ribosomes such that PKI is placed in the P site, so that the A site contains the first codon that is directly accessible for decoding without prior eEF2-mediated translocation of PKI. |
リンク | Cell Rep / PubMed:33296660 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.49 - 3.6 Å |
構造データ | EMDB-11459, PDB-6zvk: EMDB-11590, PDB-7a01: |
化合物 | ChemComp-ZN: |
由来 |
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キーワード | TRANSLATION / RIBOSOME INTERNAL RIBOSOME ENTRY SITE RNA DICISTROVIRUS Halastavi arva virus Intergenic region pseudoknot CrPV IGR IRES SERBP1 / RIBOSOME / Dicistrovirus Halastavi arva virus Intergenic region Internal ribosome entry site IRES RNA pseudoknot ribosome SERBP1 CrPV IGR IRES |