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-Structure paper
タイトル | A partial atomic structure for the flagellar hook of Salmonella typhimurium. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 102, Issue 4, Page 1023-1028, Year 2005 |
掲載日 | 2005年1月25日 |
著者 | Tanvir R Shaikh / Dennis R Thomas / James Z Chen / Fadel A Samatey / Hideyuki Matsunami / Katsumi Imada / Keiichi Namba / David J Derosier / |
PubMed 要旨 | The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- ...The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- and C-terminal sequences of the eight axial proteins were predicted to form interlocking alpha-domains generating an axial tube. We report on an approximately 1-nm resolution map of the hook from Salmonella typhimurium, which reveals such a tube made from interdigitated, 1-nm rod-like densities similar to those seen in maps of the filament. Atomic models for the two outer domains of the hook subunit were docked into the corresponding outermost features of the map. The N and C termini of the hook subunit fragment are positioned next to each other and face toward the axis of the hook. The placement of these termini would permit the residues missing in the fragment to form the rod-like features that form the core domain of the hook. We also fit the hook atomic model to an approximately 2-nm resolution map of the hook from Caulobacter crescentus. The hook protein sequence from C. crescentus is largely homologous to that of S. typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook. |
リンク | Proc Natl Acad Sci U S A / PubMed:15657146 / PubMed Central |
手法 | EM (らせん対称) / EM (電子線結晶学) |
解像度 | 9.0 Å |
構造データ | EMDB-1132: A partial atomic structure for the flagellar hook of Salmonella typhimurium. |
由来 |
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キーワード | MOTOR PROTEIN / BACTERIAL MOTILITY / FLAGELLAR HOOK / FLAGELLUM / BACTERIAL MOTIILITY / BACTERIAL FLAGELLAR HOOK |