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-Structure paper
タイトル | Structural insights into Fe-S protein biogenesis by the CIA targeting complex. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 27, Issue 8, Page 735-742, Year 2020 |
掲載日 | 2020年7月6日 |
著者 | Susanne A Kassube / Nicolas H Thomä / |
PubMed 要旨 | The cytosolic iron-sulfur (Fe-S) assembly (CIA) pathway is required for the insertion of Fe-S clusters into cytosolic and nuclear client proteins, including many DNA replication and repair factors. ...The cytosolic iron-sulfur (Fe-S) assembly (CIA) pathway is required for the insertion of Fe-S clusters into cytosolic and nuclear client proteins, including many DNA replication and repair factors. The molecular mechanisms of client protein recognition and Fe-S cluster transfer remain unknown. Here, we report crystal structures of the CIA targeting complex (CTC), revealing that its CIAO2B subunit is centrally located and bridges CIAO1 and the client adaptor protein MMS19. Cryo-EM reconstructions of human CTC bound either to the DNA replication factor primase or to the DNA helicase DNA2, combined with biochemical, biophysical and yeast complementation assays, reveal an evolutionarily conserved, bipartite client recognition mode facilitated by CIAO1 and the structural flexibility of the MMS19 subunit. Unexpectedly, the primase Fe-S cluster is located ~70 Å away from the CTC reactive cysteine, implicating conformational dynamics of the CTC or additional maturation factors in the mechanism of Fe-S cluster transfer. |
リンク | Nat Struct Mol Biol / PubMed:32632277 |
手法 | EM (単粒子) / X線回折 |
解像度 | 2 - 15.8 Å |
構造データ | EMDB-11016: EMDB-11017: EMDB-11018: EMDB-11019: EMDB-11020: EMDB-11021: PDB-6tbl: PDB-6tbn: PDB-6tc0: |
化合物 | ChemComp-K: ChemComp-EDO: ChemComp-HOH: ChemComp-NA: |
由来 |
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キーワード | BIOSYNTHETIC PROTEIN / Fe-S cluster / Fe-S / CIA / CIA targeting complex / CIA core complex / CYTOSOLIC PROTEIN |