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-Structure paper
タイトル | The Ccr4-Not complex monitors the translating ribosome for codon optimality. |
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ジャーナル・号・ページ | Science, Vol. 368, Issue 6488, Year 2020 |
掲載日 | 2020年4月17日 |
著者 | Robert Buschauer / Yoshitaka Matsuo / Takato Sugiyama / Ying-Hsin Chen / Najwa Alhusaini / Thomas Sweet / Ken Ikeuchi / Jingdong Cheng / Yasuko Matsuki / Risa Nobuta / Andrea Gilmozzi / Otto Berninghausen / Petr Tesina / Thomas Becker / Jeff Coller / Toshifumi Inada / Roland Beckmann / |
PubMed 要旨 | Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA ...Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability. |
リンク | Science / PubMed:32299921 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 - 3.1 Å |
構造データ | EMDB-10431, PDB-6tb3: EMDB-10537, PDB-6tnu: |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-SPD: ChemComp-3HE: |
由来 |
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キーワード | TRANSLATION / Not5 / CCR4-NOT complex / translation control / eIF5A |