EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structural basis for inhibition of ribosomal translocation by viomycin.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 117, Issue 19, Page 10271-10277, Year 2020
掲載日	2020年5月12日
著者	Ling Zhang / Ying-Hui Wang / Xing Zhang / Laura Lancaster / Jie Zhou / Harry F Noller /
PubMed 要旨	Viomycin, an antibiotic that has been used to fight tuberculosis infections, is believed to block the translocation step of protein synthesis by inhibiting ribosomal subunit dissociation and trapping ...Viomycin, an antibiotic that has been used to fight tuberculosis infections, is believed to block the translocation step of protein synthesis by inhibiting ribosomal subunit dissociation and trapping the ribosome in an intermediate state of intersubunit rotation. The mechanism by which viomycin stabilizes this state remains unexplained. To address this, we have determined cryo-EM and X-ray crystal structures of 70S ribosome complexes trapped in a rotated state by viomycin. The 3.8-Å resolution cryo-EM structure reveals a ribosome trapped in the hybrid state with 8.6° intersubunit rotation and 5.3° rotation of the 30S subunit head domain, bearing a single P/E state transfer RNA (tRNA). We identify five different binding sites for viomycin, four of which have not been previously described. To resolve the details of their binding interactions, we solved the 3.1-Å crystal structure of a viomycin-bound ribosome complex, revealing that all five viomycins bind to ribosomal RNA. One of these (Vio1) corresponds to the single viomycin that was previously identified in a complex with a nonrotated classical-state ribosome. Three of the newly observed binding sites (Vio3, Vio4, and Vio5) are clustered at intersubunit bridges, consistent with the ability of viomycin to inhibit subunit dissociation. We propose that one or more of these same three viomycins induce intersubunit rotation by selectively binding the rotated state of the ribosome at dynamic elements of 16S and 23S rRNA, thus, blocking conformational changes associated with molecular movements that are required for translocation.
リンク	Proc Natl Acad Sci U S A / PubMed:32341159 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.1 - 3.7 Å
構造データ	EMDB-0939: The Structural Basis for Inhibition of Ribosome Translocation by Viomycin 手法: EM (単粒子) / 解像度: 3.7 Å PDB-6lkq: The Structural Basis for Inhibition of Ribosomal Translocation by Viomycin 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å
由来	escherichia coli (大腸菌) synthetic construct (人工物)
キーワード	TRANSLATION / 70S ribosome viomycin translocation