EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Superimmunity by pan-sarbecovirus nanobodies.
ジャーナル・号・ページ	Cell Rep, Vol. 39, Issue 13, Page 111004, Year 2022
掲載日	2022年6月28日
著者	Yufei Xiang / Wei Huang / Hejun Liu / Zhe Sang / Sham Nambulli / Jérôme Tubiana / Kevin L Williams / W Paul Duprex / Dina Schneidman-Duhovny / Ian A Wilson / Derek J Taylor / Yi Shi /
PubMed 要旨	Vaccine boosters and infection can facilitate the development of SARS-CoV-2 antibodies with improved potency and breadth. Here, we observe superimmunity in a camelid extensively immunized with the ...Vaccine boosters and infection can facilitate the development of SARS-CoV-2 antibodies with improved potency and breadth. Here, we observe superimmunity in a camelid extensively immunized with the SARS-CoV-2 receptor-binding domain (RBD). We rapidly isolate a large repertoire of specific ultra-high-affinity nanobodies that bind strongly to all known sarbecovirus clades using integrative proteomics. These pan-sarbecovirus nanobodies (psNbs) are highly effective against SARS-CoV and SARS-CoV-2 variants, including Omicron, with the best median neutralization potency at single-digit nanograms per milliliter. A highly potent, inhalable, and bispecific psNb (PiN-31) is also developed. Structural determinations of 13 psNbs with the SARS-CoV-2 spike or RBD reveal five epitope classes, providing insights into the mechanisms and evolution of their broad activities. The highly evolved psNbs target small, flat, and flexible epitopes that contain over 75% of conserved RBD surface residues. Their potencies are strongly and negatively correlated with the distance of the epitopes from the receptor binding sites.
リンク	Cell Rep / PubMed:35738279 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.71 - 3.6 Å
構造データ	27061 8cxn EMDB-27061, PDB-8cxn: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 2-57 手法: EM (単粒子) / 解像度: 2.9 Å 27067 8cxq EMDB-27067, PDB-8cxq: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 1-22 手法: EM (単粒子) / 解像度: 2.3 Å 27068 8cy6 EMDB-27068, PDB-8cy6: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 2-65 手法: EM (単粒子) / 解像度: 3.2 Å 27069 8cy7 EMDB-27069, PDB-8cy7: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 2-34 手法: EM (単粒子) / 解像度: 2.9 Å 27071 8cy9 EMDB-27071, PDB-8cy9: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 1-23 手法: EM (単粒子) / 解像度: 2.9 Å 27072 8cya EMDB-27072, PDB-8cya: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 2-67 手法: EM (単粒子) / 解像度: 2.7 Å 27073 8cyb EMDB-27073, PDB-8cyb: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 1-8 手法: EM (単粒子) / 解像度: 2.7 Å 27074 8cyc EMDB-27074, PDB-8cyc: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 2-34 手法: EM (単粒子) / 解像度: 2.9 Å 27075 8cyd EMDB-27075, PDB-8cyd: SARS-CoV-2 Spike protein in complex with a pan-sarbecovirus nanobody 2-45 手法: EM (単粒子) / 解像度: 2.6 Å 27080 8cyj EMDB-27080, PDB-8cyj: RBD of SARS-CoV-2 Spike protein in complex with pan-sarbecovirus nanobodies 2-10, 2-67, 2-62 and 1-25 手法: EM (単粒子) / 解像度: 3.6 Å PDB-8cwu: Crystal structure of SARS-CoV-2 spike protein receptor-binding domain in complex with a cross-neutralizing nanobody 1-21 手法: X-RAY DIFFRACTION / 解像度: 1.71 Å PDB-8cwv: Crystal structure of SARS-CoV-2 spike protein receptor-binding domain in complex with a cross-neutralizing nanobody 2-31 and a human antibody CC12.1 Fab 手法: X-RAY DIFFRACTION / 解像度: 2.51 Å
化合物	ChemComp-HOH: WATER / 水 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-AH2: 1-deoxy-alpha-D-mannopyranose / 1,5-アンヒドロ-D-マンニト-ル
由来	lama glama (ラマ) severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM (免疫系) / cross-neutralizing antibody; nanobody; SARS-CoV-2; Sarbecovirus; IMMUNE SYSTEM; VHH / cross-neutralizing antibody; nanobody; SARS-CoV-2; Sarbecovirus; IMMUNE SYSTEM; VHH; CC12.1 / VIRAL PROTEIN (ウイルスタンパク質) / SARS-CoV-2 (SARSコロナウイルス2) / Spike protein (スパイクタンパク質) / nanobody (ナノボディ) / broad binding