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-Structure paper
タイトル | Bidirectional contraction of a type six secretion system. |
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ジャーナル・号・ページ | Nat Commun, Vol. 10, Issue 1, Page 1565, Year 2019 |
掲載日 | 2019年4月5日 |
著者 | Piotr Szwedziak / Martin Pilhofer / |
PubMed 要旨 | Contractile injection systems (CISs) mediate cell-cell interactions by a phage tail-like apparatus. Their conserved mechanism relies on the anchoring of the proximal end of a sheath-tube module to a ...Contractile injection systems (CISs) mediate cell-cell interactions by a phage tail-like apparatus. Their conserved mechanism relies on the anchoring of the proximal end of a sheath-tube module to a membrane, followed by contraction of the sheath towards the attachment site and ejection of the inner tube. Here we reveal a major variation of the CIS mechanism in the type six secretion system (T6SS) of enteroaggregative Escherichia coli (EAEC). We show that both ends of the sheath-tube module are attached to opposite sides of the cell, enabling the structure to contract in two opposite directions. The protein TssA1 mediates the interaction of the distal end with the cell envelope, the termination of tail elongation, and non-canonical contraction towards the distal end. We provide a framework for the molecular processes at the T6SS distal end. Further research will address whether bidirectional contraction allows for bidirectional effector secretion. The unrecognized concept of non-canonical contractions could be relevant to biofilms of the human intestine. |
リンク | Nat Commun / PubMed:30952865 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-4694: EMDB-4695: EMDB-4696: EMDB-4697: |
由来 |
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