EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the native Sec61 protein-conducting channel.
ジャーナル・号・ページ	Nat Commun, Vol. 6, Page 8403, Year 2015
掲載日	2015年9月28日
著者	Stefan Pfeffer / Laura Burbaum / Pia Unverdorben / Markus Pech / Yuxiang Chen / Richard Zimmermann / Roland Beckmann / Friedrich Förster /
PubMed 要旨	In mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, ...In mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, which has been structurally studied in isolated, detergent-solubilized states. Here we structurally and functionally characterize native, non-solubilized ribosome-Sec61 complexes on rough ER vesicles using cryo-electron tomography and ribosome profiling. Surprisingly, the 9-Å resolution subtomogram average reveals Sec61 in a laterally open conformation, even though the channel is not in the process of inserting membrane proteins into the lipid bilayer. In contrast to recent mechanistic models for polypeptide translocation and insertion, our results indicate that the laterally open conformation of Sec61 is the only conformation present in the ribosome-bound translocon complex, independent of its functional state. Consistent with earlier functional studies, our structure suggests that the ribosome alone, even without a nascent chain, is sufficient for lateral opening of Sec61 in a lipid environment.
リンク	Nat Commun / PubMed:26411746 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	9.0 - 10.0 Å
構造データ	3068 5a6u EMDB-3068: Mammalian ribosome bound to the native Sec61 protein-conducting channel in the 'non-inserting' state ('conventional' alignment) PDB-5a6u: Native mammalian ribosome-bound Sec61 protein-conducting channel in the 'non-inserting' state 手法: EM (サブトモグラム平均) / 解像度: 9.0 Å EMDB-3069: Mammalian ribosome bound to the native Sec61 protein-conducting channel in the 'non-inserting' state ('gold standard' alignment) 手法: EM (サブトモグラム平均) / 解像度: 9.0 Å EMDB-3070: Mammalian ribosome bound to the native Sec61 protein-conducting channel in the idle state 手法: EM (サブトモグラム平均) / 解像度: 9.4 Å EMDB-3071: Mammalian ribosome bound to the native Sec61 protein-conducting channel in the translocating state 手法: EM (サブトモグラム平均) / 解像度: 10.0 Å EMDB-3072: Representative tomogram of canine pancreatic rough Endoplasmic Reticulum vesicles 手法: EM (トモグラフィー)
由来	canis lupus familiaris (イヌ)
キーワード	TRANSLATION (翻訳 (生物学)) / RIBOSOME (リボソーム) / SEC61 (Sec61) / TRANSLOCON (トランスロコン) / ENDOPLASMIC RETICULUM (小胞体) / CRYOELECTRON TOMOGRAPHY / SUBTOMOGRAM ANALYSIS