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TitleTMPRSS2 and glycan receptors synergistically facilitate coronavirus entry.
Journal, issue, pagesCell, Vol. 187, Issue 16, Page 4261-4271.e17, Year 2024
Publish dateAug 8, 2024
AuthorsHaofeng Wang / Xiaoce Liu / Xiang Zhang / Zhuoqian Zhao / Yuchi Lu / Dingzhe Pu / Zeyang Zhang / Jie Chen / Yajie Wang / Mengfei Li / Xuxue Dong / Yinkai Duan / Yujia He / Qiyu Mao / Hangtian Guo / Haoran Sun / Yihan Zhou / Qi Yang / Yan Gao / Xiuna Yang / Hongzhi Cao / Luke Guddat / Lei Sun / Zihe Rao / Haitao Yang /
PubMed AbstractThe entry of coronaviruses is initiated by spike recognition of host cellular receptors, involving proteinaceous and/or glycan receptors. Recently, TMPRSS2 was identified as the proteinaceous ...The entry of coronaviruses is initiated by spike recognition of host cellular receptors, involving proteinaceous and/or glycan receptors. Recently, TMPRSS2 was identified as the proteinaceous receptor for HCoV-HKU1 alongside sialoglycan as a glycan receptor. However, the underlying mechanisms for viral entry remain unknown. Here, we investigated the HCoV-HKU1C spike in the inactive, glycan-activated, and functionally anchored states, revealing that sialoglycan binding induces a conformational change of the NTD and promotes the neighboring RBD of the spike to open for TMPRSS2 recognition, exhibiting a synergistic mechanism for the entry of HCoV-HKU1. The RBD of HCoV-HKU1 features an insertion subdomain that recognizes TMPRSS2 through three previously undiscovered interfaces. Furthermore, structural investigation of HCoV-HKU1A in combination with mutagenesis and binding assays confirms a conserved receptor recognition pattern adopted by HCoV-HKU1. These studies advance our understanding of the complex viral-host interactions during entry, laying the groundwork for developing new therapeutics against coronavirus-associated diseases.
External linksCell / PubMed:38964329
MethodsEM (single particle)
Resolution2.95 - 3.65 Å
Structure data

EMDB-39025, PDB-8y7x:
Structure of HCoV-HKU1A spike in the functionally anchored-3up conformation with 3TMPRSS2
Method: EM (single particle) / Resolution: 3.09 Å

EMDB-39026, PDB-8y7y:
Local structure of HCoV-HKU1A spike in complex with TMPRSS2 and glycan
Method: EM (single particle) / Resolution: 3.24 Å

EMDB-39036, PDB-8y87:
Structure of HCoV-HKU1C spike in the functionally anchored-1up conformation with 1TMPRSS2
Method: EM (single particle) / Resolution: 3.26 Å

EMDB-39037, PDB-8y88:
Structure of HCoV-HKU1C spike in the functionally anchored-2up conformation with 2TMPRSS2
Method: EM (single particle) / Resolution: 3.03 Å

EMDB-39038, PDB-8y89:
Structure of HCoV-HKU1C spike in the functionally anchored-3up conformation with 2TMPRSS2
Method: EM (single particle) / Resolution: 3.32 Å

EMDB-39039, PDB-8y8a:
Structure of HCoV-HKU1C spike in the functionally anchored-3up conformation with 3TMPRSS2
Method: EM (single particle) / Resolution: 3.19 Å

EMDB-39040, PDB-8y8b:
Local structure of HCoV-HKU1C spike in complex with TMPRSS2 and glycan
Method: EM (single particle) / Resolution: 3.01 Å

EMDB-39041, PDB-8y8c:
Structure of HCoV-HKU1C spike in the inactive-closed conformation
Method: EM (single particle) / Resolution: 2.95 Å

EMDB-39042, PDB-8y8d:
Structure of HCoV-HKU1C spike in the inactive-1up conformation
Method: EM (single particle) / Resolution: 3.41 Å

EMDB-39043, PDB-8y8e:
Structure of HCoV-HKU1C spike in the inactive-2up conformation
Method: EM (single particle) / Resolution: 3.62 Å

EMDB-39044, PDB-8y8f:
Structure of HCoV-HKU1C spike in the glycan-activated-closed conformation
Method: EM (single particle) / Resolution: 3.07 Å

EMDB-39045, PDB-8y8g:
Structure of HCoV-HKU1C spike in the glycan-activated-1up conformation
Method: EM (single particle) / Resolution: 3.23 Å

EMDB-39046, PDB-8y8h:
Structure of HCoV-HKU1C spike in the glycan-activated-2up conformation
Method: EM (single particle) / Resolution: 3.65 Å

EMDB-39047, PDB-8y8i:
Structure of HCoV-HKU1C spike in the glycan-activated-3up conformation
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-39048, PDB-8y8j:
Local structure of HCoV-HKU1C spike in complex with glycan
Method: EM (single particle) / Resolution: 3.57 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-MJJ:
methyl 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosidonic acid

Source
  • human coronavirus hku1
  • human coronavirus hku1 (isolate n1)
  • homo sapiens (human)
  • human coronavirus hku1 (isolate n5)
KeywordsVIRAL PROTEIN / HKU1A / spike / TMPRSS2 / HKU1C

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