+Search query
-Structure paper
Title | Functionalized graphene grids with various charges for single-particle cryo-EM. |
---|---|
Journal, issue, pages | Nat Commun, Vol. 13, Issue 1, Page 6718, Year 2022 |
Publish date | Nov 7, 2022 |
Authors | Ye Lu / Nan Liu / Yongbo Liu / Liming Zheng / Junhao Yang / Jia Wang / Xia Jia / Qinru Zi / Hailin Peng / Yu Rao / Hong-Wei Wang / |
PubMed Abstract | A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In ...A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination. |
External links | Nat Commun / PubMed:36344519 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.2 Å |
Structure data | PDB-8h2h: |
Source |
|
Keywords | RNA BINDING PROTEIN/RNA / intron / RNA BINDING PROTEIN-RNA COMPLEX |