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TitleStructural insights into actin isoforms.
Journal, issue, pagesElife, Vol. 12, Year 2023
Publish dateFeb 15, 2023
AuthorsAmandeep S Arora / Hsiang-Ling Huang / Ramanpreet Singh / Yoshie Narui / Andrejus Suchenko / Tomoyuki Hatano / Sarah M Heissler / Mohan K Balasubramanian / Krishna Chinthalapudi /
PubMed AbstractActin isoforms organize into distinct networks that are essential for the normal function of eukaryotic cells. Despite a high level of sequence and structure conservation, subtle differences in their ...Actin isoforms organize into distinct networks that are essential for the normal function of eukaryotic cells. Despite a high level of sequence and structure conservation, subtle differences in their design principles determine the interaction with myosin motors and actin-binding proteins. Therefore, identifying how the structure of actin isoforms relates to function is important for our understanding of normal cytoskeletal physiology. Here, we report the high-resolution structures of filamentous skeletal muscle α-actin (3.37 Å), cardiac muscle α-actin (3.07 Å), ß-actin (2.99 Å), and γ-actin (3.38 Å) in the Mg·ADP state with their native post-translational modifications. The structures revealed isoform-specific conformations of the N-terminus that shift closer to the filament surface upon myosin binding, thereby establishing isoform-specific interfaces. Collectively, the structures of single-isotype, post-translationally modified bare skeletal muscle α-actin, cardiac muscle α-actin, ß-actin, and γ-actin reveal general principles, similarities, and differences between isoforms. They complement the repertoire of known actin structures and allow for a comprehensive understanding of in vitro and in vivo functions of actin isoforms.
External linksElife / PubMed:36790143 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution2.99 - 3.38 Å
Structure data

27548

8dmx

EMDB-27548, PDB-8dmx:
Cryo-EM structure of skeletal muscle alpha-actin
Method: EM (helical sym.) / Resolution: 3.37 Å

27549

8dmy

EMDB-27549, PDB-8dmy:
Cryo-EM structure of cardiac muscle alpha-actin
Method: EM (single particle) / Resolution: 3.07 Å

27565

8dnf

EMDB-27565, PDB-8dnf:
Cryo-EM structure of nonmuscle gamma-actin
Method: EM (single particle) / Resolution: 3.38 Å

27572

8dnh

EMDB-27572, PDB-8dnh:
Cryo-EM structure of nonmuscle beta-actin
Method: EM (single particle) / Resolution: 2.99 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • oryctolagus cuniculus (rabbit)
  • rabbit (rabbit)
  • bos taurus (cattle)
  • cattle (cattle)
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / cytoskeleton

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