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Structure paper

TitleArchitecture of the Heme-translocating CcmABCD/E complex required for Cytochrome c maturation.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5190, Year 2023
Publish dateAug 25, 2023
AuthorsLorena Ilcu / Lukas Denkhaus / Anton Brausemann / Lin Zhang / Oliver Einsle /
PubMed AbstractMono- and multiheme cytochromes c are post-translationally matured by the covalent attachment of heme. For this, Escherichia coli employs the most complex type of maturation machineries, the Ccm- ...Mono- and multiheme cytochromes c are post-translationally matured by the covalent attachment of heme. For this, Escherichia coli employs the most complex type of maturation machineries, the Ccm-system (for cytochrome c maturation). It consists of two membrane protein complexes, one of which shuttles heme across the membrane to a mobile chaperone that then delivers the cofactor to the second complex, an apoprotein:heme lyase, for covalent attachment. Here we report cryo-electron microscopic structures of the heme translocation complex CcmABCD from E. coli, alone and bound to the heme chaperone CcmE. CcmABCD forms a heterooctameric complex centered around the ABC transporter CcmAB that does not by itself transport heme. Our data suggest that the complex flops a heme group from the inner to the outer leaflet at its CcmBC interfaces, driven by ATP hydrolysis at CcmA. A conserved heme-handling motif (WxWD) at the periplasmic side of CcmC rotates the heme by 90° for covalent attachment to the heme chaperone CcmE that we find interacting exclusively with the CcmB subunit.
External linksNat Commun / PubMed:37626034 / PubMed Central
MethodsEM (single particle)
Resolution3.47 - 3.94 Å
Structure data

16597

8ce1

EMDB-16597, PDB-8ce1:
Cytochrome c maturation complex CcmABCD
Method: EM (single particle) / Resolution: 3.47 Å

16599

8ce5

EMDB-16599, PDB-8ce5:
Cytochrome c maturation complex CcmABCD, E154Q, ATP-bound
Method: EM (single particle) / Resolution: 3.62 Å

16601

8ce8

EMDB-16601, PDB-8ce8:
Cytochrome c maturation complex CcmABCDE
Method: EM (single particle) / Resolution: 3.81 Å

16602

8cea

EMDB-16602, PDB-8cea:
Cytochrome c maturation complex CcmABCD, E154Q
Method: EM (single particle) / Resolution: 3.94 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • escherichia coli (E. coli)
  • escherichia coli k-12 (bacteria)
KeywordsMEMBRANE PROTEIN / Cytochrome c maturation

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