Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM captures early ribosome assembly in action.
Journal, issue, pages	Nat Commun, Vol. 14, Issue 1, Page 898, Year 2023
Publish date	Feb 17, 2023
Authors	Bo Qin / Simon M Lauer / Annika Balke / Carlos H Vieira-Vieira / Jörg Bürger / Thorsten Mielke / Matthias Selbach / Patrick Scheerer / Christian M T Spahn / Rainer Nikolay /
PubMed Abstract	Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal ...Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal proteins. To obtain insights into the still unexplored early assembly phase of the bacterial 50S subunit, we exploited a minimal in vitro reconstitution system using purified ribosomal components and scalable reaction conditions. Time-limited assembly assays combined with cryo-EM analysis visualizes the structurally complex assembly pathway starting with a particle consisting of ordered density for only ~500 nucleotides of 23S rRNA domain I and three ribosomal proteins. In addition, our structural analysis reveals that early 50S assembly occurs in a domain-wise fashion, while late 50S assembly proceeds incrementally. Furthermore, we find that both ribosomal proteins and folded rRNA helices, occupying surface exposed regions on pre-50S particles, induce, or stabilize rRNA folds within adjacent regions, thereby creating cooperativity.
External links	Nat Commun / PubMed:36797249 / PubMed Central
Methods	EM (single particle)
Resolution	3.03 - 6.62 Å
Structure data	16494 8c8x EMDB-16494, PDB-8c8x: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.93 Å 16495 8c8y EMDB-16495, PDB-8c8y: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.03 Å 16496 8c8z EMDB-16496, PDB-8c8z: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.12 Å 16497 8c90 EMDB-16497, PDB-8c90: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.15 Å 16498 8c91 EMDB-16498, PDB-8c91: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 4.19 Å 16499 8c92 EMDB-16499, PDB-8c92: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.79 Å 16500 8c93 EMDB-16500, PDB-8c93: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 4.17 Å 16501 8c94 EMDB-16501, PDB-8c94: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.8 Å 16502 8c95 EMDB-16502, PDB-8c95: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 4.92 Å 16503 8c96 EMDB-16503, PDB-8c96: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 4.43 Å 16504 8c97 EMDB-16504, PDB-8c97: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 4.07 Å 16505 8c98 EMDB-16505, PDB-8c98: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.66 Å 16506 8c99 EMDB-16506, PDB-8c99: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 3.29 Å 16507 8c9a EMDB-16507, PDB-8c9a: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 4.86 Å 16508 8c9b EMDB-16508, PDB-8c9b: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 5.9 Å 16509 8c9c EMDB-16509, PDB-8c9c: Cryo-EM captures early ribosome assembly in action Method: EM (single particle) / Resolution: 6.62 Å
Source	escherichia coli (E. coli)
Keywords	RIBOSOME / ribosome assembly / ribosome biogenesis / total reconstitution / RNA / ribosomal protein.