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-Structure paper
Title | Structural basis for intrinsic transcription termination. |
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Journal, issue, pages | Nature, Vol. 613, Issue 7945, Page 783-789, Year 2023 |
Publish date | Jan 11, 2023 |
Authors | Linlin You / Expery O Omollo / Chengzhi Yu / Rachel A Mooney / Jing Shi / Liqiang Shen / Xiaoxian Wu / Aijia Wen / Dingwei He / Yuan Zeng / Yu Feng / Robert Landick / Yu Zhang / |
PubMed Abstract | Efficient and accurate termination is required for gene transcription in all living organisms. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a ...Efficient and accurate termination is required for gene transcription in all living organisms. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway-called intrinsic termination transcription in bacteria-in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of Escherichia coli transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases. |
External links | Nature / PubMed:36631609 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.05 - 3.58 Å |
Structure data | EMDB-33996, PDB-7yp9: EMDB-33997, PDB-7ypa: EMDB-33998, PDB-7ypb: |
Chemicals | ChemComp-ZN: ChemComp-MG: |
Source |
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Keywords | TRANSCRIPTION/DNA/RNA / Termination / Intrinsic termination / U-tract pause / Transcription pause / Factor-independent termination / TRANSCRIPTION-DNA-RNA COMPLEX / Hairpin nucleation / Pre-termination / TRANSCRIPTION//DNA/RNA / RNA release / Transcription bubble rewinding / TRANSCRIPTION--DNA-RNA complex |