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TitleMolecular basis for the selective G protein signaling of somatostatin receptors.
Journal, issue, pagesNat Chem Biol, Vol. 19, Issue 2, Page 133-140, Year 2023
Publish dateSep 22, 2022
AuthorsSijia Chen / Xiao Teng / Sanduo Zheng /
PubMed AbstractG protein-coupled receptors (GPCRs) modulate every aspect of physiological functions mainly through activating heterotrimeric G proteins. A majority of GPCRs promiscuously couple to multiple G ...G protein-coupled receptors (GPCRs) modulate every aspect of physiological functions mainly through activating heterotrimeric G proteins. A majority of GPCRs promiscuously couple to multiple G protein subtypes. Here we validate that in addition to the well-known G pathway, somatostatin receptor 2 and 5 (SSTR2 and SSTR5) couple to the G pathway and show that smaller ligands preferentially activate the G pathway. We further determined cryo-electron microscopy structures of the SSTR2‒G and SSTR2‒G complexes bound to octreotide and SST-14. Structural and functional analysis revealed that G protein selectivity of SSTRs is not only determined by structural elements in the receptor-G protein interface, but also by the conformation of the agonist-binding pocket. Accordingly, smaller ligands fail to stabilize a broader agonist-binding pocket of SSTRs that is required for efficient G coupling but not G coupling. Our studies facilitate the design of drugs with selective G protein signaling to improve therapeutic efficacy.
External linksNat Chem Biol / PubMed:36138141
MethodsEM (single particle)
Resolution3.25 - 3.48 Å
Structure data

33585

7y24

EMDB-33585, PDB-7y24:
Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex
Method: EM (single particle) / Resolution: 3.25 Å

33586

7y26

EMDB-33586, PDB-7y26:
Cryo-EM structure of the octreotide-bound SSTR2-miniGq-scFv16 complex
Method: EM (single particle) / Resolution: 3.3 Å

33587

7y27

EMDB-33587, PDB-7y27:
Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex
Method: EM (single particle) / Resolution: 3.48 Å

Chemicals

ChemComp-CLR:
CHOLESTEROL

Source
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / GPCR / somatostatin receptor / G protein / biased ligand

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