Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike.
Journal, issue, pages	PLoS Pathog, Vol. 18, Issue 7, Page e1010583, Year 2022
Publish date	Jul 29, 2022
Authors	Kun Qu / Qiuluan Chen / Katarzyna A Ciazynska / Banghui Liu / Xixi Zhang / Jingjing Wang / Yujie He / Jiali Guan / Jun He / Tian Liu / Xiaofei Zhang / Andrew P Carter / Xiaoli Xiong / John A G Briggs /
PubMed Abstract	The spike (S) protein of SARS-CoV-2 has been observed in three distinct pre-fusion conformations: locked, closed and open. Of these, the function of the locked conformation remains poorly understood. ...The spike (S) protein of SARS-CoV-2 has been observed in three distinct pre-fusion conformations: locked, closed and open. Of these, the function of the locked conformation remains poorly understood. Here we engineered a SARS-CoV-2 S protein construct "S-R/x3" to arrest SARS-CoV-2 spikes in the locked conformation by a disulfide bond. Using this construct we determined high-resolution structures confirming that the x3 disulfide bond has the ability to stabilize the otherwise transient locked conformations. Structural analyses reveal that wild-type SARS-CoV-2 spike can adopt two distinct locked-1 and locked-2 conformations. For the D614G spike, based on which all variants of concern were evolved, only the locked-2 conformation was observed. Analysis of the structures suggests that rigidified domain D in the locked conformations interacts with the hinge to domain C and thereby restrains RBD movement. Structural change in domain D correlates with spike conformational change. We propose that the locked-1 and locked-2 conformations of S are present in the acidic high-lipid cellular compartments during virus assembly and egress. In this model, release of the virion into the neutral pH extracellular space would favour transition to the closed or open conformations. The dynamics of this transition can be altered by mutations that modulate domain D structure, as is the case for the D614G mutation, leading to changes in viral fitness. The S-R/x3 construct provides a tool for the further structural and functional characterization of the locked conformations of S, as well as how sequence changes might alter S assembly and regulation of receptor binding domain dynamics.
External links	PLoS Pathog / PubMed:35905112 / PubMed Central
Methods	EM (single particle)
Resolution	2.6 - 3.5 Å
Structure data	33453 7xtz EMDB-33453, PDB-7xtz: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Locked-1 Conformation Method: EM (single particle) / Resolution: 2.8 Å 33454 7xu0 EMDB-33454, PDB-7xu0: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Locked-211 Conformation Method: EM (single particle) / Resolution: 2.9 Å 33455 7xu1 EMDB-33455, PDB-7xu1: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Locked-122 Conformation Method: EM (single particle) / Resolution: 3.0 Å 33456 7xu2 EMDB-33456, PDB-7xu2: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Locked-2 Conformation Method: EM (single particle) / Resolution: 3.2 Å 33457 7xu3 EMDB-33457, PDB-7xu3: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Closed Conformation Method: EM (single particle) / Resolution: 3.0 Å 33458 7xu4 EMDB-33458, PDB-7xu4: Structure of SARS-CoV-2 D614G Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Locked-2 Conformation Method: EM (single particle) / Resolution: 3.2 Å 33459 7xu5 EMDB-33459, PDB-7xu5: Structure of SARS-CoV-2 D614G Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), Closed Conformation Method: EM (single particle) / Resolution: 3.1 Å 33460 7xu6 EMDB-33460, PDB-7xu6: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), incubated in Low pH after 40-Day Storage in PBS, Locked-2 Conformation Method: EM (single particle) / Resolution: 2.9 Å EMDB-33461: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site) after 40-Day Storage in PBS, Closed Conformation Method: EM (single particle) / Resolution: 2.6 Å EMDB-33462: Structure of SARS-CoV-2 Spike Protein with Engineered x3 Disulfide (x3(D427C, V987C) and single Arg S1/S2 cleavage site), incubated in Low pH after 40-Day Storage in PBS, Closed Conformation Method: EM (single particle) / Resolution: 3.5 Å EMDB-33463: Structure of SARS-CoV-2 D614G spike protein (single Arg S1/S2 cleavage site), Closed Conformation Method: EM (single particle) / Resolution: 3.0 Å EMDB-33464: Structure of SARS-Cov-2 D614G spike protein (single Arg S1/S2 cleavage site), Open Conformation Method: EM (single particle) / Resolution: 3.1 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-BLA: BILIVERDINE IX ALPHA ChemComp-EIC: LINOLEIC ACID
Source	severe acute respiratory syndrome coronavirus 2
Keywords	VIRAL PROTEIN / protein engineering / spike protein / SARS-CoV-2