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TitleStructural basis of leukotriene B4 receptor 1 activation.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 1156, Year 2022
Publish dateMar 3, 2022
AuthorsNa Wang / Xinheng He / Jing Zhao / Hualiang Jiang / Xi Cheng / Yu Xia / H Eric Xu / Yuanzheng He /
PubMed AbstractLeukotriene B4 receptor 1 (BLT1) plays crucial roles in the acute inflammatory responses and is a valuable target for anti-inflammation treatment, however, the mechanism by which leukotriene B4 (LTB4) ...Leukotriene B4 receptor 1 (BLT1) plays crucial roles in the acute inflammatory responses and is a valuable target for anti-inflammation treatment, however, the mechanism by which leukotriene B4 (LTB4) activates receptor remains unclear. Here, we report the cryo-electron microscopy (cryo-EM) structure of the LTB4 -bound human BLT1 in complex with a G protein in an active conformation at resolution of 2.91 Å. In combination of molecule dynamics (MD) simulation, docking and site-directed mutagenesis, our structure reveals that a hydrogen-bond network of water molecules and key polar residues is the key molecular determinant for LTB4 binding. We also find that the displacement of residues M101 and I271 to the center of receptor, which unlock the ion lock of the lower part of pocket, is the key mechanism of receptor activation. In addition, we reveal a binding site of phosphatidylinositol (PI) and discover that the widely open ligand binding pocket may contribute the lack of specificity and efficacy for current BLT1-targeting drug design. Taken together, our structural analysis provides a scaffold for understanding BLT1 activation and a rational basis for designing anti-leukotriene drugs.
External linksNat Commun / PubMed:35241677 / PubMed Central
MethodsEM (single particle)
Resolution2.9 Å
Structure data

32018

7vkt

EMDB-32018, PDB-7vkt:
cryo-EM structure of LTB4-bound BLT1 in complex with Gi protein
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-LTB:
LEUKOTRIENE B4

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-8IO:
[(2R)-2-[(Z)-hexadec-9-enoyl]oxy-3-[oxidanyl-[(2S,3R,5R,6S)-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl]oxy-phosphoryl]oxy-propyl] octadecanoate

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / GPCR

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