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Structure paper

TitleHigh-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateNov 23, 2021
AuthorsSabrina Pospich / H Lee Sweeney / Anne Houdusse / Stefan Raunser /
PubMed AbstractThe molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are ...The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin.
External linksElife / PubMed:34812732 / PubMed Central
MethodsEM (helical sym.)
Resolution2.9 - 3.7 Å
Structure data

EMDB-13501, PDB-7plt:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er)
Method: EM (helical sym.) / Resolution: 3.3 Å

EMDB-13502, PDB-7plu:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er)
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-13503, PDB-7plv:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 1)
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-13504, PDB-7plw:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 2)
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-13505, PDB-7plx:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4)
Method: EM (helical sym.) / Resolution: 3.6 Å

EMDB-13506, PDB-7ply:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin)
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-13507, PDB-7plz:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er, young JASP-stabilized F-actin)
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-13508, PDB-7pm0:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 1)
Method: EM (helical sym.) / Resolution: 3.6 Å

EMDB-13509, PDB-7pm1:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 2)
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-13510, PDB-7pm2:
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 4)
Method: EM (helical sym.) / Resolution: 3.6 Å

EMDB-13511, PDB-7pm3:
Cryo-EM structure of young JASP-stabilized F-actin (central 3er)
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-13521, PDB-7pm5:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er)
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-13522, PDB-7pm6:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 3er/2er)
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-13523, PDB-7pm7:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 2)
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-13524, PDB-7pm8:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 3)
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-13525, PDB-7pm9:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 4)
Method: EM (helical sym.) / Resolution: 3.7 Å

EMDB-13526, PDB-7pma:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 5)
Method: EM (helical sym.) / Resolution: 3.6 Å

EMDB-13527, PDB-7pmb:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 6)
Method: EM (helical sym.) / Resolution: 3.6 Å

EMDB-13528, PDB-7pmc:
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 7)
Method: EM (helical sym.) / Resolution: 3.7 Å

EMDB-13529, PDB-7pmd:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er)
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-13530, PDB-7pme:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 3er/2er)
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-13531, PDB-7pmf:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 1)
Method: EM (helical sym.) / Resolution: 3.4 Å

EMDB-13532, PDB-7pmg:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 3)
Method: EM (helical sym.) / Resolution: 3.3 Å

EMDB-13533, PDB-7pmh:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 4)
Method: EM (helical sym.) / Resolution: 3.4 Å

EMDB-13535, PDB-7pmi:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 5)
Method: EM (helical sym.) / Resolution: 3.3 Å

EMDB-13536, PDB-7pmj:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 6)
Method: EM (helical sym.) / Resolution: 3.4 Å

EMDB-13538, PDB-7pml:
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 8)
Method: EM (helical sym.) / Resolution: 3.3 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-9UE:
Jasplakinolide

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • gallus gallus (chicken)
  • Rabbit (rabbit)
  • amanita phalloides (death cap)
  • homo sapiens (human)
  • oryctolagus cuniculus (rabbit)
  • synthetic construct (others)
KeywordsMOTOR PROTEIN / myosin / cytoskeleton / F-actin / phalloidin / jasplakinolide / STRUCTURAL PROTEIN

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