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Structure paper

TitleA ferredoxin bridge connects the two arms of plant mitochondrial complex I.
Journal, issue, pagesPlant Cell, Vol. 33, Issue 6, Page 2072-2091, Year 2021
Publish dateJul 19, 2021
AuthorsNiklas Klusch / Jennifer Senkler / Özkan Yildiz / Werner Kühlbrandt / Hans-Peter Braun /
PubMed AbstractMitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two ...Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two arms, which form a conserved L-shape. We report the structures of the intact, 47-subunit mitochondrial complex I from Arabidopsis thaliana and the 51-subunit complex I from the green alga Polytomella sp., both at around 2.9 Å resolution. In both complexes, a heterotrimeric γ-carbonic anhydrase domain is attached to the membrane arm on the matrix side. Two states are resolved in A. thaliana complex I, with different angles between the two arms and different conformations of the ND1 (NADH dehydrogenase subunit 1) loop near the quinol binding site. The angle appears to depend on a bridge domain, which links the peripheral arm to the membrane arm and includes an unusual ferredoxin. We propose that the bridge domain participates in regulating the activity of plant complex I.
External linksPlant Cell / PubMed:33768254 / PubMed Central
MethodsEM (single particle)
Resolution2.88 - 3.72 Å
Structure data

EMDB-11872, PDB-7aqq:
Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane core)
Method: EM (single particle) / Resolution: 3.06 Å

EMDB-11873, PDB-7aqr:
Cryo-EM structure of Arabidopsis thaliana Complex-I (peripheral arm)
Method: EM (single particle) / Resolution: 2.91 Å

EMDB-11874, PDB-7aqw:
Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane tip)
Method: EM (single particle) / Resolution: 3.17 Å

EMDB-11875, PDB-7ar7:
Cryo-EM structure of Arabidopsis thaliana complex-I (open conformation)
Method: EM (single particle) / Resolution: 3.72 Å

EMDB-11876, PDB-7ar8:
Cryo-EM structure of Arabidopsis thaliana complex-I (closed conformation)
Method: EM (single particle) / Resolution: 3.53 Å

EMDB-11877, PDB-7ar9:
Cryo-EM structure of Polytomella Complex-I (membrane arm)
Method: EM (single particle) / Resolution: 2.97 Å

EMDB-11878, PDB-7arb:
Cryo-EM structure of Arabidopsis thaliana Complex-I (complete composition)
Method: EM (single particle) / Resolution: 3.41 Å

EMDB-11879, PDB-7arc:
Cryo-EM structure of Polytomella Complex-I (peripheral arm)
Method: EM (single particle) / Resolution: 2.88 Å

EMDB-11880, PDB-7ard:
Cryo-EM structure of Polytomella Complex-I (complete composition)
Method: EM (single particle) / Resolution: 3.11 Å

Chemicals

ChemComp-UQ9:
Ubiquinone-9

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

ChemComp-LMN:
Lauryl Maltose Neopentyl Glycol / detergent*YM

ChemComp-FE:
Unknown entry

ChemComp-PC7:
(7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / phospholipid*YM

ChemComp-ZN:
Unknown entry

ChemComp-PGT:
(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / phospholipid*YM

ChemComp-PSF:
1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE

ChemComp-T7X:
Phosphatidylinositol

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-8Q1:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-HOH:
WATER

Source
  • arabidopsis thaliana (thale cress)
  • Mouse-ear cress (thale cress)
  • thale cress (thale cress)
  • polytomella sp. pringsheim 198.80 (plant)
KeywordsELECTRON TRANSPORT / Complex-I / Complex-I Arabidopsis

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