+Search query
-Structure paper
Title | Structure of a human 48 translational initiation complex. |
---|---|
Journal, issue, pages | Science, Vol. 369, Issue 6508, Page 1220-1227, Year 2020 |
Publish date | Sep 4, 2020 |
Authors | Jailson Brito Querido / Masaaki Sokabe / Sebastian Kraatz / Yuliya Gordiyenko / J Mark Skehel / Christopher S Fraser / V Ramakrishnan / |
PubMed Abstract | A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to ...A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning. |
External links | Science / PubMed:32883864 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 6.0 Å |
Structure data | EMDB-10769, PDB-6ybd: EMDB-10772, PDB-6ybs: EMDB-10773, PDB-6ybt: EMDB-10774, PDB-6ybv: EMDB-10775, PDB-6ybw: EMDB-11302, PDB-6zmw: |
Chemicals | ChemComp-MG: ChemComp-ZN: |
Source |
|
Keywords | TRANSLATION / eIF3 / ribosome / initiation complex |