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TitleThe Ccr4-Not complex monitors the translating ribosome for codon optimality.
Journal, issue, pagesScience, Vol. 368, Issue 6488, Year 2020
Publish dateApr 17, 2020
AuthorsRobert Buschauer / Yoshitaka Matsuo / Takato Sugiyama / Ying-Hsin Chen / Najwa Alhusaini / Thomas Sweet / Ken Ikeuchi / Jingdong Cheng / Yasuko Matsuki / Risa Nobuta / Andrea Gilmozzi / Otto Berninghausen / Petr Tesina / Thomas Becker / Jeff Coller / Toshifumi Inada / Roland Beckmann /
PubMed AbstractControl of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA ...Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability.
External linksScience / PubMed:32299921 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.1 Å
Structure data

10431

6tb3

EMDB-10431, PDB-6tb3:
yeast 80S ribosome in complex with the Not5 subunit of the CCR4-NOT complex
Method: EM (single particle) / Resolution: 2.8 Å

10537

6tnu

EMDB-10537, PDB-6tnu:
Yeast 80S ribosome in complex with eIF5A and decoding A-site and P-site tRNAs.
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-SPD:
SPERMIDINE

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSLATION / Not5 / CCR4-NOT complex / translation control / eIF5A

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