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TitleCueR activates transcription through a DNA distortion mechanism.
Journal, issue, pagesNat Chem Biol, Vol. 17, Issue 1, Page 57-64, Year 2021
Publish dateSep 28, 2020
AuthorsChengli Fang / Steven J Philips / Xiaoxian Wu / Kui Chen / Jing Shi / Liqiang Shen / Juncao Xu / Yu Feng / Thomas V O'Halloran / Yu Zhang /
PubMed AbstractThe MerR-family transcription factors (TFs) are a large group of bacterial proteins responding to cellular metal ions and multiple antibiotics by binding within central RNA polymerase-binding regions ...The MerR-family transcription factors (TFs) are a large group of bacterial proteins responding to cellular metal ions and multiple antibiotics by binding within central RNA polymerase-binding regions of a promoter. While most TFs alter transcription through protein-protein interactions, MerR TFs are capable of reshaping promoter DNA. To address the question of which mechanism prevails, we determined two cryo-EM structures of transcription activation complexes (TAC) comprising Escherichia coli CueR (a prototype MerR TF), RNAP holoenzyme and promoter DNA. The structures reveal that this TF promotes productive promoter-polymerase association without canonical protein-protein contacts seen between other activator proteins and RNAP. Instead, CueR realigns the key promoter elements in the transcription activation complex by clamp-like protein-DNA interactions: these induce four distinct kinks that ultimately position the -10 element for formation of the transcription bubble. These structural and biochemical results provide strong support for the DNA distortion paradigm of allosteric transcriptional control by MerR TFs.
External linksNat Chem Biol / PubMed:32989300 / PubMed Central
MethodsEM (single particle)
Resolution3.69 - 4.22 Å
Structure data

0874

6ldi

EMDB-0874, PDB-6ldi:
The cryo-EM structure of E. coli CueR transcription activation complex
Method: EM (single particle) / Resolution: 3.69 Å

30268

7c17

EMDB-30268, PDB-7c17:
The cryo-EM structure of E. coli CueR transcription activation complex with fully duplex promoter DNA
Method: EM (single particle) / Resolution: 4.22 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-AG:
SILVER ION

Source
  • Escherichia coli K-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
  • synthetic construct (others)
KeywordsTRANSCRIPTION (DNA to RNA) / RNA polymerase / CueR / transcription activation / TRANSCRIPTION

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