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Structure paper

TitleSnapshots of the Catalytic Cycle of an O2, Pyridoxal Phosphate-Dependent Hydroxylase.
Journal, issue, pagesACS Chem. Biol., Vol. 13, Page 965-974, Year 2018
Publish dateJan 9, 2018 (structure data deposition date)
AuthorsHedges, J.B. / Kuatsjah, E. / Du, Y.L. / Eltis, L.D. / Ryan, K.S.
External linksACS Chem. Biol. / PubMed:29466666
MethodsX-ray diffraction
Resolution1.5 - 1.55 Å
Structure data

PDB-6c3a:
O2-, PLP-dependent L-arginine hydroxylase RohP 4-hydroxy-2-ketoarginine complex
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-6c3b:
O2-, PLP-Dependent L-Arginine Hydroxylase RohP Holoenzyme
Method: X-RAY DIFFRACTION / Resolution: 1.51 Å

PDB-6c3c:
PLP-dependent L-arginine hydroxylase RohP quinonoid I complex
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6c3d:
O2-, PLP-dependent L-arginine hydroxylase RohP quinonoid II complex
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-EGV:
(4S)-5-carbamimidamido-4-hydroxy-2-oxopentanoic acid

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EJ1:
(2E)-5-carbamimidamido-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}pentanoic acid

ChemComp-EMS:
(2E,3E)-5-carbamimidamido-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}pent-3-enoic acid

Source
  • streptomyces cattleya (strain atcc 35852 / dsm 46488 / jcm 4925 / nbrc 14057 / nrrl 8057) (bacteria)
  • streptomyces cattleya (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / O2- / PLP-dependent hydroxylase / PLP-dependent L-arginine hydroxylase

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