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TitleCryo EM structure of intact rotary H-ATPase/synthase from Thermus thermophilus.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 89, Year 2018
Publish dateJan 8, 2018
AuthorsAtsuko Nakanishi / Jun-Ichi Kishikawa / Masatada Tamakoshi / Kaoru Mitsuoka / Ken Yokoyama /
PubMed AbstractProton translocating rotary ATPases couple ATP hydrolysis/synthesis, which occurs in the soluble domain, with proton flow through the membrane domain via a rotation of the common central rotor ...Proton translocating rotary ATPases couple ATP hydrolysis/synthesis, which occurs in the soluble domain, with proton flow through the membrane domain via a rotation of the common central rotor complex against the surrounding peripheral stator apparatus. Here, we present a large data set of single particle cryo-electron micrograph images of the V/A type H-rotary ATPase from the bacterium Thermus thermophilus, enabling the identification of three rotational states based on the orientation of the rotor subunit. Using masked refinement and classification with signal subtractions, we obtain homogeneous reconstructions for the whole complexes and soluble V domains. These reconstructions are of higher resolution than any EM map of intact rotary ATPase reported previously, providing a detailed molecular basis for how the rotary ATPase maintains structural integrity of the peripheral stator apparatus, and confirming the existence of a clear proton translocation path from both sides of the membrane.
External linksNat Commun / PubMed:29311594 / PubMed Central
MethodsEM (single particle)
Resolution4.7 - 7.5 Å
Structure data

6810

5y5x

EMDB-6810, PDB-5y5x:
V/A-type ATPase/synthase from Thermus thermophilus, rotational state 1
Method: EM (single particle) / Resolution: 5.0 Å

6811

5y5y

EMDB-6811, PDB-5y5y:
V/A-type ATPase/synthase from Thermus thermophilus, peripheral domain, rotational state 1
Method: EM (single particle) / Resolution: 4.7 Å

6812

5y5z

EMDB-6812, PDB-5y5z:
V/A-type ATPase/synthase from Thermus thermophilus, rotational state 2
Method: EM (single particle) / Resolution: 6.7 Å

6813

5y60

EMDB-6813, PDB-5y60:
V/A-type ATPase/synthase from Thermus thermophilus, rotational state 3.
Method: EM (single particle) / Resolution: 7.5 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • thermus thermophilus hb8 (bacteria)
KeywordsMOTOR PROTEIN / ATP synthase / proton pump / V-ATPase / Bioenergetics

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