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Structure paper

TitleStructures and characterization of digoxin- and bufalin-bound Na+,K+-ATPase compared with the ouabain-bound complex.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 112, Page 1755-1760, Year 2015
Publish dateSep 23, 2014 (structure data deposition date)
AuthorsLaursen, M. / Gregersen, J.L. / Yatime, L. / Nissen, P. / Fedosova, N.U.
External linksProc. Natl. Acad. Sci. USA / PubMed:25624492
MethodsX-ray diffraction
Resolution3.408 - 4 Å
Structure data

PDB-4res:
Crystal structure of the Na,K-ATPase E2P-bufalin complex with bound potassium
Method: X-RAY DIFFRACTION / Resolution: 3.408 Å

PDB-4ret:
Crystal structure of the Na,K-ATPase E2P-digoxin complex with bound magnesium
Method: X-RAY DIFFRACTION / Resolution: 4 Å

Chemicals

ChemComp-BUF:
bufalin / toxin*YM

ChemComp-CLR:
CHOLESTEROL

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-17F:
O-[(S)-({(2R)-2,3-bis[(9Z)-octadec-9-enoyloxy]propyl}oxy)(hydroxy)phosphoryl]-L-serine / phospholipid*YM

ChemComp-DGX:
DIGOXIN / medication*YM

ChemComp-HOH:
WATER

Source
  • sus scrofa (pig)
KeywordsMEMBRANE PROTEIN / HYDROLASE/INHIBITOR / alpha-helical transmembrane protein / ATPase / sodium ion transport / potassium ion transport / ATP binding / sodium binding / potassium binding / receptor for cardiotonic steroids / phosphorylation / glycosylation / plasma membrane / multisubunit complex / trimeric complex / HYDROLASE-INHIBITOR complex

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