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TitleCryo-EM structures of two bovine adenovirus type 3 intermediates.
Journal, issue, pagesVirology, Vol. 450-451, Page 174-181, Year 2014
Publish dateJan 3, 2014
AuthorsLingpeng Cheng / Xiaoxing Huang / Xiaomin Li / Wei Xiong / Wei Sun / Chongwen Yang / Kai Zhang / Ying Wang / Hongrong Liu / Xiaojun Huang / Gang Ji / Fei Sun / Congyi Zheng / Ping Zhu /
PubMed AbstractAdenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained ...Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical. The overall facet structure of BAd3 exhibits a similar structure to human Ads; however, BAd3 protein IX has a unique conformation. Mass spectrometry and cryo-electron tomography analyses indicate that one intermediate structure represents the stage during DNA encapsidation, whilst the other intermediate structure represents a later stage. These results also suggest that cleavage of precursor protein VI occurs during, rather than after, the DNA encapsidation process. Overall, our results provide insights into the mechanism of Ad assembly, and allow the first structural comparison between human and nonhuman Ads at backbone level.
External linksVirology / PubMed:24503080
MethodsEM (single particle)
Resolution4.5 Å
Structure data

EMDB-2272:
Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly
Method: EM (single particle) / Resolution: 4.5 Å

2273

3zif

EMDB-2273, PDB-3zif:
Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly
Method: EM (single particle) / Resolution: 4.5 Å

Source
  • bovine adenovirus 3
KeywordsVIRUS / ASSEMBLY INTERMEDIATE

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