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TitleDiscovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site.
Journal, issue, pagesNat Commun, Vol. 10, Page 2222-2222, Year 2019
Publish dateNov 12, 2013 (structure data deposition date)
AuthorsStreltsov, V.A. / Luang, S. / Peisley, A. / Varghese, J.N. / Ketudat Cairns, J.R. / Fort, S. / Hijnen, M. / Tvaroska, I. / Arda, A. / Jimenez-Barbero, J. ...Streltsov, V.A. / Luang, S. / Peisley, A. / Varghese, J.N. / Ketudat Cairns, J.R. / Fort, S. / Hijnen, M. / Tvaroska, I. / Arda, A. / Jimenez-Barbero, J. / Alfonso-Prieto, M. / Rovira, C. / Mendoza, F. / Tiessler-Sala, L. / Sanchez-Aparicio, J.E. / Rodriguez-Guerra, J. / Lluch, J.M. / Marechal, J.D. / Masgrau, L. / Hrmova, M.
External linksNat Commun / PubMed:31110237
MethodsX-ray diffraction
Resolution1.45 - 2.3 Å
Structure data

PDB-3wlh:
Crystal Structure Analysis of Plant Exohydrolase
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-3wli:
Crystal Structure Analysis of Plant Exohydrolase
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-3wlj:
Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 3-deoxy-glucose
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

PDB-3wlk:
Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 4-deoxy-glucose
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3wll:
Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with PEG400
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3wlm:
Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with octyl-O-glucoside
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3wln:
Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with octyl-S-glucoside
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3wlo:
Crystal Structure Analysis of Plant Exohydrolase
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-3wlp:
Crystal Structure Analysis of Plant Exohydrolase
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-3wlq:
Crystal Structure Analysis of Plant Exohydrolase
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-3wlr:
Crystal Structure Analysis of Plant Exohydrolase
Method: X-RAY DIFFRACTION / Resolution: 2.21 Å

PDB-6md6:
CRYSTAL STRUCTURE ANALYSIS OF PLANT EXOHYDROLASE IN COMPLEX WITH METHYL 2-THIO-BETA-SOPHOROSIDE
Method: X-RAY DIFFRACTION / Resolution: 1.68 Å

PDB-6mi1:
CRYSTAL STRUCTURE ANALYSIS OF THE VARIANT PLANT EXOHYDROLASE ARG158ALA-GLU161ALA IN COMPLEX WITH METHYL 6-THIO-BETA-GENTIOBIOSIDE
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-3DO:
3-deoxy-beta-D-glucopyranose

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-BOG:
octyl beta-D-glucopyranoside / detergent*YM

ChemComp-SOG:
octyl 1-thio-beta-D-glucopyranoside / detergent*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

Source
  • hordeum vulgare subsp. vulgare (domesticated barley)
KeywordsHYDROLASE / BETA BARREL / GRAIN DEVELOPMENT / Enzyme Function Initiative / Tim Barrel/Beta sheet / N-glycosylation / plant apoplast

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