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TitleSeven cysteine-deficient mutants depict the interplay between thermal and chemical stabilities of individual cysteine residues in mitogen-activated protein kinase c-Jun N-terminal kinase 1
Journal, issue, pagesBiochemistry, Vol. 51, Page 8410-8421, Year 2012
Publish dateJun 28, 2012 (structure data deposition date)
AuthorsNakaniwa, T. / Fukada, H. / Inoue, T. / Gouda, M. / Nakai, R. / Kirii, Y. / Adachi, M. / Tamada, T. / Segawa, S. / Kuroki, R. ...Nakaniwa, T. / Fukada, H. / Inoue, T. / Gouda, M. / Nakai, R. / Kirii, Y. / Adachi, M. / Tamada, T. / Segawa, S. / Kuroki, R. / Tada, T. / Kinoshita, T.
External linksBiochemistry / PubMed:23020677
MethodsX-ray diffraction
Resolution2.69 - 3.5 Å
Structure data

PDB-3vud:
Crystal structure of a cysteine-deficient mutant M1 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

PDB-3vug:
Crystal structure of a cysteine-deficient mutant M2 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 3.24 Å

PDB-3vuh:
Crystal structure of a cysteine-deficient mutant M3 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-3vui:
Crystal structure of a cysteine-deficient mutant M2 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-3vuk:
Crystal structure of a cysteine-deficient mutant M5 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-3vul:
Crystal structure of a cysteine-deficient mutant M1 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 2.81 Å

PDB-3vum:
Crystal structure of a cysteine-deficient mutant M7 in MAP kinase JNK1
Method: X-RAY DIFFRACTION / Resolution: 2.69 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • homo sapiens (human)
KeywordsTRANSFERASE/TRANSFERASE INHIBITOR / MAP kinase / Kinase domain / phosphorylation / ATP Binding / TRANSFERASE-TRANSFERASE INHIBITOR complex / TRANSCRIPTION

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