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Structure paper

TitleStructural basis for substrate binding and regioselective oxidation of monosaccharides at c3 by pyranose 2-oxidase.
Journal, issue, pagesJ. Biol. Chem., Vol. 281, Page 35104-35115, Year 2006
Publish dateSep 22, 2006 (structure data deposition date)
AuthorsKujawa, M. / Ebner, H. / Leitner, C. / Hallberg, B.M. / Prongjit, M. / Sucharitakul, J. / Ludwig, R. / Rudsander, U. / Peterbauer, C. / Chaiyen, P. ...Kujawa, M. / Ebner, H. / Leitner, C. / Hallberg, B.M. / Prongjit, M. / Sucharitakul, J. / Ludwig, R. / Rudsander, U. / Peterbauer, C. / Chaiyen, P. / Haltrich, D. / Divne, C.
External linksJ. Biol. Chem. / PubMed:16984920
MethodsX-ray diffraction
Resolution1.65 - 1.95 Å
Structure data

PDB-2igk:
Crystal structure of recombinant pyranose 2-oxidase
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2igm:
Crystal structure of recombinant pyranose 2-oxidase H548N mutant
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-2ign:
Crystal structure of recombinant pyranose 2-oxidase H167A mutant
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-2igo:
Crystal structure of pyranose 2-oxidase H167A mutant with 2-fluoro-2-deoxy-D-glucose
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-SHG:
2-deoxy-2-fluoro-beta-D-glucopyranose

Source
  • trametes ochracea (fungus)
KeywordsOXIDOREDUCTASE / GMC OXIDOREDUCTASE / ROSSMANN FOLD / PHBH FOLD / HOMOTETRAMER / 8-ALPHA-(N3) HISTIDYL FLAVINYLATION / ACTIVE-SITE MUTANT / H548N / H167A MUTANT / 2-FLUORO-2-DEOXY-D-GLUCOSE

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