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Structure paper

TitleStructure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and NAD+
Journal, issue, pagesProteins, Vol. 70, Page 517-527, Year 2008
Publish dateSep 20, 2005 (structure data deposition date)
AuthorsNango, E. / Kumasaka, T. / Hirayama, T. / Tanaka, N. / Eguchi, T.
External linksProteins / PubMed:17879343
MethodsX-ray diffraction
Resolution2.15 - 2.3 Å
Structure data

PDB-2d2x:
Crystal structure of 2-deoxy-scyllo-inosose synthase
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-2gru:
Crystal structure of 2-deoxy-scyllo-inosose synthase complexed with carbaglucose-6-phosphate, NAD+ and Co2+
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-CO:
Unknown entry

ChemComp-SO3:
SULFITE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-EXO:
(1R,2S,3S,4R)-5-METHYLENECYCLOHEXANE-1,2,3,4-TETRAOL

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-CAK:
[(1R,2R,3S,4S,5R)-2,3,4,5-TETRAHYDROXYCYCLOHEXYL]METHYL DIHYDROGEN PHOSPHATE

Source
  • bacillus circulans (bacteria)
KeywordsLYASE / aminoglycoside / 2-deoxystreptamine / dehydroquinate synthase

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