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-Structure paper
Title | Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle. |
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Journal, issue, pages | J Cell Biol, Vol. 150, Issue 3, Page 447-460, Year 2000 |
Publish date | Aug 7, 2000 |
Authors | R K Agrawal / C M Spahn / P Penczek / R A Grassucci / K H Nierhaus / J Frank / |
PubMed Abstract | Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the ...Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible. |
External links | J Cell Biol / PubMed:10931859 / PubMed Central |
Methods | EM (single particle) |
Resolution | 17 Å |
Structure data | PDB-1fcw: |
Source |
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Keywords | RIBOSOME / tRNA binding sites / protein synthesis / elongation cycle |