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-Structure paper
Title | Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism. |
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Journal, issue, pages | Elife, Vol. 8, Year 2019 |
Publish date | Feb 11, 2019 |
Authors | Sam Li / Jose-Jesus Fernandez / Wallace F Marshall / David A Agard / |
PubMed Abstract | Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the ...Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from . We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. The structure infers that POC1 is likely an integral component of A-C linker. Its conserved WD40 β-propeller domain provides attachment sites for other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly. |
External links | Elife / PubMed:30741631 / PubMed Central |
Methods | EM (subtomogram averaging) |
Resolution | 21.4 - 23.1 Å |
Structure data | EMDB-9167: EMDB-9168: EMDB-9169: EMDB-9170: EMDB-9171: EMDB-9172: EMDB-9173: EMDB-9174: |
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