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TitleInsight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis.
Journal, issue, pagesVirology, Vol. 485, Page 171-178, Year 2015
Publish dateAug 11, 2015
AuthorsChun-Yan Wang / Qin-Fen Zhang / Yuan-Zhu Gao / Xue-Ping Zhou / Gang Ji / Xiao-Jun Huang / Jian Hong / Chuan-Xi Zhang /
PubMed AbstractMaize chlorotic mottle virus (MCMV) is the only member of the Machlomovirus genus in the family Tombusviridae. Here, we obtained the Cryo-EM structure of MCMV by single particle analysis with most ...Maize chlorotic mottle virus (MCMV) is the only member of the Machlomovirus genus in the family Tombusviridae. Here, we obtained the Cryo-EM structure of MCMV by single particle analysis with most local resolution at approximately 4 Å. The Cα backbone was built based on residues with bulky side chains. The resolved C-terminus of the capsid protein subunit and obvious openings at the 2-fold axis demonstrated the compactness of the asymmetric unit, which indicates an important role in the stability of MCMV. The Asp116 residue from each subunit around the 5-fold and 3-fold axes contributed to the negative charges in the centers of the pentamers and hexamers, which might serve as a solid barrier against the leakage of genomic RNA. Finally, the loops most exposed on the surface were analyzed and are proposed to be potential functional sites related to MCMV transmission.
External linksVirology / PubMed:26275511
MethodsEM (single particle)
Resolution3.6 Å
Structure data

EMDB-6382: Three-dimensional structure of Maize Chlorotic Mottle Virus by Single Particle Analysis
PDB-3jb8: Insight into Three-dimensional structure of Maize Chlorotic Mottle Virus Revealed by Single Particle Analysis
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • maize chlorotic mottle virus
KeywordsVIRUS / MCMV / Single Particle Analysis / Three-dimensional Structure / icosahedral virus / Transmission

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