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TitleA Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease.
Journal, issue, pagesSci Rep, Vol. 5, Page 14440, Year 2015
Publish dateSep 23, 2015
AuthorsBrian L Gilmore / Carly E Winton / Andrew C Demmert / Justin R Tanner / Sam Bowman / Vasilea Karageorge / Kaya Patel / Zhi Sheng / Deborah F Kelly /
PubMed AbstractWe present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable ...We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.
External linksSci Rep / PubMed:26395823 / PubMed Central
MethodsEM (single particle)
Resolution22.0 Å
Structure data

EMDB-6340:
Electron cryo-microscopy of a BRCA1-RNAPII transcriptional complex
Method: EM (single particle) / Resolution: 22.0 Å

Source
  • Homo sapiens (human)

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